Cas9 deactivation with photocleavable guide RNAs
نویسندگان
چکیده
منابع مشابه
Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.
The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous t...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2021
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2021.02.007